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metadata.dc.type: article
Título : Effects of accelerated ageing and cryopreservation on seeds and embryonic axes of Phaseolus vulgaris L and Arachis hypogaea L. Germination and seedlings vigor
Autor : Pérez Ruiz, César Félix
Palabras clave : EGUMINOSEAE;PHASEOLUS VULGARIS;ARACHIS HYPOGAEA;ARTIFICIAL AGING;CRYOPRESERVATION;GERMINATION;SEEDLINGS VIGOR;ELECTROLYTE LEAKAGE
Fecha de publicación : 2017
Citación : Tacán, M., Tapia, C., and Pérez, C. (2017). Effects of accelerated ageing and cryopreservation on seeds and embryonic axes of Phaseolus vulgaris L and Arachis hypogaea L. Germination and seedlings vigor. PeerJ Preprints 5:e3203v1
Citación : DOI;https://doi.org/10.7287/peerj.preprints.3203v1
Resumen : The effects of artificial aging and cryopreservation methods on the germination of seeds and embryonic axes of Phaseolus vulgaris and Arachis hypogaea , were studied. Aging and cryopreservation treatments in bean seeds not affect the germination percentage, reaching values not significantly different from control. Germination percentage was higher when isolated embryonic axes were studied. Aging treatments gave rise to larger bean seedlings than the controls and cryopreservation treatments affected the size depending on the explant used. In groundnut seeds, aging treatments and cryopreservation positively affected germination and vigor of seedlings, but embryonic axes from not rehydrated aging seeds not germinate. Undried groundnut seeds not survive cryopreservation, while the dried ones showed a germination behavior superior to the control. Embryonic axes tolerate immersion in liquid nitrogen but when droplet-vitrification was used, formed callus instead of seedlings. Electrolyte leakage was always higher in embryonic axes than in seeds, both in beans and groundnut, and not to have a significant effect, under the conditions studied, on germination and vigor of seedlings. The present study evidences the possibility of cryopreserving, by simple procedures, germplasm of beans and groundnut, and to verify the aging tolerance of the seeds and embryonic axes of these important species.
metadata.dc.description.uri: https://peerj.com/preprints/3203/
URI : http://repositorio.educacionsuperior.gob.ec/handle/28000/5106
ISSN : 21678359
Aparece en las colecciones: Proyecto Prometeo

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